Is Annatto Gluten Free?
Yes, annatto is generally considered gluten-free. It is a natural food coloring derived from the seeds of the achiote tree and does not contain gluten-containing ingredients.

Rich in antioxidants: Annatto contains carotenoids like bixin and norbixin, which help protect cells from damage caused by free radicals.
Anti-inflammatory properties: The compounds in annatto may help reduce inflammation in the body.
Potential antimicrobial effects: Some studies suggest annatto may have antibacterial and antifungal properties.
Heart health: Annatto may help lower cholesterol levels and support cardiovascular health.
Eye health: The carotenoids in annatto can contribute to better eye health and may help prevent age-related macular degeneration.
Digestive health: Annatto has been traditionally used to aid digestion and relieve stomach discomfort.
Potential anticancer properties: Some research indicates that annatto compounds may have anticancer effects, though more studies are needed.
Bone health: Annatto contains calcium, which is essential for strong bones and teeth.
Skin health: The antioxidants in annatto may help protect skin from UV damage and signs of aging.
Blood sugar regulation: Some studies suggest annatto may help regulate blood sugar levels, potentially benefiting those with diabetes.
Liver support: Annatto may have hepatoprotective properties, potentially supporting liver health.Remember that while these benefits are promising, more research is needed to fully understand the extent of annatto’s health effects. Always consult with a healthcare professional before using annatto for medicinal purposes.
Those with known allergies to annatto or related plants: Some people may have allergic reactions to annatto, ranging from mild to severe.
Individuals with IgE-mediated food allergies: Annatto may cross-react with other allergens, potentially causing issues for those with certain food allergies.
People with a history of hives or urticaria: Annatto has been linked to cases of chronic urticaria in some individuals.
Those with sensitive skin: Some people may experience skin irritation when using annatto-based products topically.
Individuals with a history of migraines: Some studies suggest annatto may trigger migraines in susceptible people.
Pregnant and breastfeeding women: While generally considered safe, there’s limited research on annatto’s effects during pregnancy and lactation, so caution is advised.
People taking certain medications: Annatto may interact with some drugs, so it’s best to consult a healthcare provider if you’re on medication.
Those with G6PD deficiency: Annatto might potentially cause hemolytic anemia in individuals with this genetic condition.
People with known sensitivities to food dyes or additives: As annatto is often used as a natural food coloring, those sensitive to food additives should be cautious.Always consult with a healthcare professional if you have concerns about consuming or using products containing annatto.
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What is Tolerase® G?
A new Approach for People with Gluten Sensitivity:
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On this page we explain why we are so enthusiastic about this supplement, supported by scientific research.

What is Gluten?
Gluten is a group of proteins found primarily in grains such as wheat, barley, and rye. These complex proteins give shape, strength, and texture to bread and other grain products. However, gluten is difficult to digest because it is rich in proline, a non-essential amino acid. This is why about 13% of the world’s population is sensitive to gluten in their diet (1).
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It is important to note that Tolerase G is designed to have optimal activity in the difficult conditions of the stomach. Its effectiveness on the digestion of gluten in the stomach and duodenum has been scientifically proven ( 1 ).
Studies have shown that a newly identified prolyl endoprotease derived from Aspergillus niger can effectively break down gluten proteins. This enzyme works optimally at a pH of 4–5, remains stable at pH 2, and is completely resistant to digestion by pepsin ( 2 ). Furthermore, research has shown that Aspergillus niger prolyl endoprotease (AN-PEP) accelerates the breakdown of gluten in the stomach so much that very little gluten reaches the duodenum ( 3 ). This suggests that co-administration of AN-PEP with a meal containing gluten could potentially eliminate gluten toxicity, allowing people to (occasionally) abandon their strict gluten-free diet ( 3 ).
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At Little Helpers we are proud of our unique partnership with the producer of Tolerase® G DSM . Together we have spent 2.5 years of research and worked tirelessly to develop and perfect our gluten digestion pill, which contains 100% Tolerase® G.
Our shared commitment to quality and effectiveness has resulted in a product that is unmatched in the market. We have chosen to manufacture locally in Europe to ensure the highest quality standards and control.
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Little Helpers Anti-Gluten Pills contain 100% Tolerase® G. Tolerase® G is the only enzyme on the market that has been scientifically proven to be effective enough to break down gluten in the stomach to the point where the body no longer reacts to it.

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Disclaimer: We do our best to provide accurate gluten information, but errors can happen. If you spot a mistake or have concerns, please contact us so we can make it right.