Annie’s offers both gluten-free and gluten-containing mac and cheese products. To determine if a specific Annie’s mac and cheese product is gluten-free, you need to check the packaging or product information for that particular variety.

Made with organic pasta in some varieties
Contains no artificial flavors, synthetic colors, or preservatives
Uses real cheese
Some varieties are made with whole grain pasta, which provides more fiber
Fortified with calcium and protein
Lower in sodium compared to some other boxed mac and cheese brands
Some varieties are gluten-free, catering to those with gluten sensitivities
Contains some B vitamins from the enriched pasta
Can be a source of energy due to its carbohydrate content
Potentially more nutritious than some fast food options when prepared at homeIt’s important to remember that while these points highlight some positive aspects, mac and cheese should still be consumed in moderation as part of a balanced diet. It is relatively high in calories and carbohydrates, and should not be relied upon as a primary source of nutrition.
People with celiac disease or gluten sensitivity (unless using the gluten-free variety)
Individuals with dairy allergies or severe lactose intolerance
Those following a vegan or plant-based diet (unless using the vegan variety)
People on low-carb or ketogenic diets
Individuals with a corn allergy (due to potential cross-contamination)
Those with severe soy allergies (due to potential cross-contamination)
Individuals on a low-sodium diet (as processed foods often contain added salt)
People trying to avoid processed or packaged foods
Those with specific dietary restrictions prescribed by their healthcare provider
Individuals with sensitivities to artificial colors or preservatives (check the label for specific ingredients)Remember that dietary needs can vary greatly from person to person, and it’s always best to consult with a healthcare professional or registered dietitian for personalized advice.
Explore gluten management solutions like Tolerase® G.
What is Tolerase® G?
A new Approach for People with Gluten Sensitivity:
Explore the Science and Benefits of Tolerase® G.
Relief from Gluten Sensitivity
In the fight against gluten, there is a unique supplement that stands out: Little Helpers anti-gluten pills with 100% Tolerase® G.
This specific enzyme, Tolerase® G, a patented endopeptidase specific for proline, was developed by Royal DSM and has been scientifically proven to be effective in breaking down gluten residues ( 1) . Furthermore, research has shown that Aspergillus niger prolyl endoprotease (AN-PEP), an enzyme closely related to Tolerase® G, can break down gluten so effectively that it barely reaches the duodenum, potentially eliminating gluten toxicity ( 2) ( 3 ) .
On this page we explain why we are so enthusiastic about this supplement, supported by scientific research.

What is Gluten?
Gluten is a group of proteins found primarily in grains such as wheat, barley, and rye. These complex proteins give shape, strength, and texture to bread and other grain products. However, gluten is difficult to digest because it is rich in proline, a non-essential amino acid. This is why about 13% of the world’s population is sensitive to gluten in their diet (1).
How Tolerase® G Works
It is important to note that Tolerase G is designed to have optimal activity in the difficult conditions of the stomach. Its effectiveness on the digestion of gluten in the stomach and duodenum has been scientifically proven ( 1 ).
Studies have shown that a newly identified prolyl endoprotease derived from Aspergillus niger can effectively break down gluten proteins. This enzyme works optimally at a pH of 4–5, remains stable at pH 2, and is completely resistant to digestion by pepsin ( 2 ). Furthermore, research has shown that Aspergillus niger prolyl endoprotease (AN-PEP) accelerates the breakdown of gluten in the stomach so much that very little gluten reaches the duodenum ( 3 ). This suggests that co-administration of AN-PEP with a meal containing gluten could potentially eliminate gluten toxicity, allowing people to (occasionally) abandon their strict gluten-free diet ( 3 ).
100% Tolerase® G Anti-Gluten Pills

Unique Enzyme Solution
Discover how our 100% Tolerase® G pills help digest gluten and support your lifestyle in a clear video.
Little Helpers & Tolerase® G: Stronger Together in the Fight against Gluten.
At Little Helpers we are proud of our unique partnership with the producer of Tolerase® G DSM . Together we have spent 2.5 years of research and worked tirelessly to develop and perfect our gluten digestion pill, which contains 100% Tolerase® G.
Our shared commitment to quality and effectiveness has resulted in a product that is unmatched in the market. We have chosen to manufacture locally in Europe to ensure the highest quality standards and control.
Our pill has proven to be many times more effective than our competitors, and we offer it at the best price. We are convinced that our pill can make a difference for people who struggle with gluten sensitivity. That is why we encourage everyone to try it and experience the benefits for themselves!
The Only Effective Anti-Gluten Pills
Little Helpers Anti-Gluten Pills contain 100% Tolerase® G. Tolerase® G is the only enzyme on the market that has been scientifically proven to be effective enough to break down gluten in the stomach to the point where the body no longer reacts to it.

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