Is Butter Gluten Free?
Yes, pure butter is naturally gluten-free. It’s made from milk or cream and does not contain wheat, barley, rye, or any other gluten-containing ingredients.

Rich source of fat-soluble vitamins (A, D, E, and K)
Contains conjugated linoleic acid (CLA), which may have anti-cancer properties
Provides a good source of energy due to its high-fat content
Contains butyrate, a short-chain fatty acid that may support gut health
Rich in omega-3 and omega-6 fatty acids help regulate inflammation in the body.
Natural source of cholesterol, which is essential for hormone production
Contains lauric acid, which may have antimicrobial properties
Can help in the absorption of nutrients from other foods when consumed together
May help stabilize blood sugar levels when consumed as part of a balanced meal
Grass-fed butter is particularly rich in vitamin K2, which is important for bone and heart health
Butter is high in beta-carotene and may help lower your chances of cancerRemember that while butter has benefits, it is high in saturated fat and calories, so it should be consumed in moderation as part of a balanced diet.
Individuals with high cholesterol levels
People with heart disease or at high risk for cardiovascular problems
Those on a low-fat diet for medical reasons
Individuals trying to lose weight or maintain weight loss
People with dairy allergies or severe lactose intolerance
Those following a vegan lifestyle
Individuals with a history of gallbladder issues or gallstones
People with certain liver conditions
Those with a family history of heart disease (should consume in moderation)
People with certain types of inflammatory conditions that may be exacerbated by saturated fatsRemember that dietary needs can vary greatly between individuals, and it’s always best to consult with a healthcare professional or registered dietitian for personalized advice.
Explore gluten management solutions like Tolerase® G.
What is Tolerase® G?
A new Approach for People with Gluten Sensitivity:
Explore the Science and Benefits of Tolerase® G.
Relief from Gluten Sensitivity
In the fight against gluten, there is a unique supplement that stands out: Little Helpers anti-gluten pills with 100% Tolerase® G.
This specific enzyme, Tolerase® G, a patented endopeptidase specific for proline, was developed by Royal DSM and has been scientifically proven to be effective in breaking down gluten residues ( 1) . Furthermore, research has shown that Aspergillus niger prolyl endoprotease (AN-PEP), an enzyme closely related to Tolerase® G, can break down gluten so effectively that it barely reaches the duodenum, potentially eliminating gluten toxicity ( 2) ( 3 ) .
On this page we explain why we are so enthusiastic about this supplement, supported by scientific research.

What is Gluten?
Gluten is a group of proteins found primarily in grains such as wheat, barley, and rye. These complex proteins give shape, strength, and texture to bread and other grain products. However, gluten is difficult to digest because it is rich in proline, a non-essential amino acid. This is why about 13% of the world’s population is sensitive to gluten in their diet (1).
How Tolerase® G Works
It is important to note that Tolerase G is designed to have optimal activity in the difficult conditions of the stomach. Its effectiveness on the digestion of gluten in the stomach and duodenum has been scientifically proven ( 1 ).
Studies have shown that a newly identified prolyl endoprotease derived from Aspergillus niger can effectively break down gluten proteins. This enzyme works optimally at a pH of 4–5, remains stable at pH 2, and is completely resistant to digestion by pepsin ( 2 ). Furthermore, research has shown that Aspergillus niger prolyl endoprotease (AN-PEP) accelerates the breakdown of gluten in the stomach so much that very little gluten reaches the duodenum ( 3 ). This suggests that co-administration of AN-PEP with a meal containing gluten could potentially eliminate gluten toxicity, allowing people to (occasionally) abandon their strict gluten-free diet ( 3 ).
100% Tolerase® G Anti-Gluten Pills

Unique Enzyme Solution
Discover how our 100% Tolerase® G pills help digest gluten and support your lifestyle in a clear video.
Little Helpers & Tolerase® G: Stronger Together in the Fight against Gluten.
At Little Helpers we are proud of our unique partnership with the producer of Tolerase® G DSM . Together we have spent 2.5 years of research and worked tirelessly to develop and perfect our gluten digestion pill, which contains 100% Tolerase® G.
Our shared commitment to quality and effectiveness has resulted in a product that is unmatched in the market. We have chosen to manufacture locally in Europe to ensure the highest quality standards and control.
Our pill has proven to be many times more effective than our competitors, and we offer it at the best price. We are convinced that our pill can make a difference for people who struggle with gluten sensitivity. That is why we encourage everyone to try it and experience the benefits for themselves!
The Only Effective Anti-Gluten Pills
Little Helpers Anti-Gluten Pills contain 100% Tolerase® G. Tolerase® G is the only enzyme on the market that has been scientifically proven to be effective enough to break down gluten in the stomach to the point where the body no longer reacts to it.

Frequently
Asked Questions 1
Disclaimer: We do our best to provide accurate gluten information, but errors can happen. If you spot a mistake or have concerns, please contact us so we can make it right.