Is Ghee Gluten Free?
Yes, ghee is gluten-free. Ghee is a type of clarified butter that has had all milk solids removed, leaving only pure butterfat, which naturally contains no gluten.

Rich in fat-soluble vitamins: Contains vitamins A, D, E, and K
High in butyrate: A short-chain fatty acid that may support digestive health
Contains conjugated linoleic acid (CLA): May have anti-inflammatory properties
Lactose-free: Suitable for those with lactose intolerance
High smoke point: Safer for high-heat cooking compared to many other oils
May support heart health: Contains healthy fatty acids that may improve cholesterol levels
Potential anti-inflammatory properties: May help reduce inflammation in the body
Rich in antioxidants: May help protect cells from damage caused by free radicals
May boost immune function: Contains compounds that support the immune system
Supports bone health: Contains vitamin K2, which is important for bone metabolism
May aid in weight management: The fatty acids in ghee may help support healthy metabolism
Potential cognitive benefits: Contains compounds that may support brain health
May improve insulin sensitivity: Some studies suggest it could help regulate blood sugar levelsIt’s important to note that while ghee offers these potential benefits, it should be consumed in moderation as part of a balanced diet due to its high calorie and fat content.
Individuals with high cholesterol levels or at risk for heart disease
People following a low-fat diet for health reasons
Those with lactose intolerance (though ghee contains very little lactose, some may still be sensitive)
Individuals trying to lose weight or maintain a calorie-restricted diet
People with dairy allergies (though ghee has most milk proteins removed, traces may remain)
Those following a vegan lifestyle
Individuals with a history of pancreatitis or gallbladder issues
People with liver problems who need to limit fat intake
Those advised by their healthcare provider to reduce saturated fat intake
Individuals with certain genetic conditions that affect fat metabolismRemember, it’s always best to consult with a healthcare professional or registered dietitian for personalized advice based on individual health conditions and dietary needs.
Explore gluten management solutions like Tolerase® G.
What is Tolerase® G?
A new Approach for People with Gluten Sensitivity:
Explore the Science and Benefits of Tolerase® G.
Relief from Gluten Sensitivity
In the fight against gluten, there is a unique supplement that stands out: Little Helpers anti-gluten pills with 100% Tolerase® G.
This specific enzyme, Tolerase® G, a patented endopeptidase specific for proline, was developed by Royal DSM and has been scientifically proven to be effective in breaking down gluten residues ( 1) . Furthermore, research has shown that Aspergillus niger prolyl endoprotease (AN-PEP), an enzyme closely related to Tolerase® G, can break down gluten so effectively that it barely reaches the duodenum, potentially eliminating gluten toxicity ( 2) ( 3 ) .
On this page we explain why we are so enthusiastic about this supplement, supported by scientific research.

What is Gluten?
Gluten is a group of proteins found primarily in grains such as wheat, barley, and rye. These complex proteins give shape, strength, and texture to bread and other grain products. However, gluten is difficult to digest because it is rich in proline, a non-essential amino acid. This is why about 13% of the world’s population is sensitive to gluten in their diet (1).
How Tolerase® G Works
It is important to note that Tolerase G is designed to have optimal activity in the difficult conditions of the stomach. Its effectiveness on the digestion of gluten in the stomach and duodenum has been scientifically proven ( 1 ).
Studies have shown that a newly identified prolyl endoprotease derived from Aspergillus niger can effectively break down gluten proteins. This enzyme works optimally at a pH of 4–5, remains stable at pH 2, and is completely resistant to digestion by pepsin ( 2 ). Furthermore, research has shown that Aspergillus niger prolyl endoprotease (AN-PEP) accelerates the breakdown of gluten in the stomach so much that very little gluten reaches the duodenum ( 3 ). This suggests that co-administration of AN-PEP with a meal containing gluten could potentially eliminate gluten toxicity, allowing people to (occasionally) abandon their strict gluten-free diet ( 3 ).
100% Tolerase® G Anti-Gluten Pills

Unique Enzyme Solution
Discover how our 100% Tolerase® G pills help digest gluten and support your lifestyle in a clear video.
Little Helpers & Tolerase® G: Stronger Together in the Fight against Gluten.
At Little Helpers we are proud of our unique partnership with the producer of Tolerase® G DSM . Together we have spent 2.5 years of research and worked tirelessly to develop and perfect our gluten digestion pill, which contains 100% Tolerase® G.
Our shared commitment to quality and effectiveness has resulted in a product that is unmatched in the market. We have chosen to manufacture locally in Europe to ensure the highest quality standards and control.
Our pill has proven to be many times more effective than our competitors, and we offer it at the best price. We are convinced that our pill can make a difference for people who struggle with gluten sensitivity. That is why we encourage everyone to try it and experience the benefits for themselves!
The Only Effective Anti-Gluten Pills
Little Helpers Anti-Gluten Pills contain 100% Tolerase® G. Tolerase® G is the only enzyme on the market that has been scientifically proven to be effective enough to break down gluten in the stomach to the point where the body no longer reacts to it.

Frequently Asked Questions
Our Expert
Drs. Ilse van Asperen
Drs. Ilse van Asperen is a medical doctor specialising in orthomolecular therapy and nutritional coaching. With a background in public and clinical health research and management, she focuses on addressing the root causes of health issues through diet, gut health, and stress reduction. As a professional editor for Little Helpers, she reviews all gluten-free content to ensure accuracy, safety, and scientific credibility. More about me
Our Sources
The information on this page is based on reputable health and nutrition organisations, ensuring accuracy and reliability for anyone following a gluten-free diet. For further details, see the sources below.
Sources
Experiences of our customers
Disclaimer: We do our best to provide accurate gluten information, but errors can happen. If you spot a mistake or have concerns, please contact us so we can make it right.