Is Glutamate Gluten Free?
Yes, glutamate is gluten-free. Glutamate is an amino acid that is chemically unrelated to gluten, which is a protein found in wheat, barley, and rye.

Neurotransmitter function: Glutamate is the primary excitatory neurotransmitter in the brain, essential for learning, memory, and cognitive function.
Protein synthesis: As an amino acid, glutamate is crucial for building proteins in the body.
Energy production: Glutamate can be converted to α-ketoglutarate, which plays a role in the citric acid cycle for energy production.
Gut health: It supports the health and function of the intestinal lining.
Immune system support: Glutamate is important for the proper functioning of immune cells.
Flavor enhancement: As a flavor enhancer (in the form of monosodium glutamate), it can make foods more palatable, potentially encouraging better nutrition.
Antioxidant production: Glutamate is a precursor to glutathione, a powerful antioxidant in the body.
Blood sugar regulation: It may play a role in insulin secretion and glucose metabolism.
Brain development: Glutamate is crucial for proper brain development in fetuses and young children.
Detoxification: It assists in the body’s natural detoxification processes, particularly in the liver.
Wound healing: Glutamate is involved in tissue repair processes.It’s important to note that while glutamate has these benefits, excessive amounts can be harmful, particularly to brain health. Balance is key, and most people get sufficient glutamate from a normal diet.
People with glutamate sensitivity or intolerance
Individuals with migraine headaches, as glutamate may trigger or worsen symptoms
Those with asthma, as some studies suggest a potential link between glutamate and asthma exacerbation
People with epilepsy, as glutamate may affect seizure activity in some cases
Individuals with neurodegenerative disorders like Alzheimer’s or Parkinson’s disease, due to glutamate’s role in neurotransmission
Those with anxiety disorders, as glutamate may influence anxiety levels
People with certain gastrointestinal issues, such as irritable bowel syndrome (IBS)
Individuals with autism spectrum disorders, as some research suggests a potential connection between glutamate and autism symptoms
Those with chronic pain conditions, as glutamate may play a role in pain signaling
People with certain types of cancer, as glutamate may influence tumor growth in some casesIt’s important to note that while these groups may need to be cautious, individual responses to glutamate can vary. Always consult with a healthcare professional for personalized advice.
Explore gluten management solutions like Tolerase® G.
What is Tolerase® G?
A new Approach for People with Gluten Sensitivity:
Explore the Science and Benefits of Tolerase® G.
Relief from Gluten Sensitivity
In the fight against gluten, there is a unique supplement that stands out: Little Helpers anti-gluten pills with 100% Tolerase® G.
This specific enzyme, Tolerase® G, a patented endopeptidase specific for proline, was developed by Royal DSM and has been scientifically proven to be effective in breaking down gluten residues ( 1) . Furthermore, research has shown that Aspergillus niger prolyl endoprotease (AN-PEP), an enzyme closely related to Tolerase® G, can break down gluten so effectively that it barely reaches the duodenum, potentially eliminating gluten toxicity ( 2) ( 3 ) .
On this page we explain why we are so enthusiastic about this supplement, supported by scientific research.

What is Gluten?
Gluten is a group of proteins found primarily in grains such as wheat, barley, and rye. These complex proteins give shape, strength, and texture to bread and other grain products. However, gluten is difficult to digest because it is rich in proline, a non-essential amino acid. This is why about 13% of the world’s population is sensitive to gluten in their diet (1).
How Tolerase® G Works
It is important to note that Tolerase G is designed to have optimal activity in the difficult conditions of the stomach. Its effectiveness on the digestion of gluten in the stomach and duodenum has been scientifically proven ( 1 ).
Studies have shown that a newly identified prolyl endoprotease derived from Aspergillus niger can effectively break down gluten proteins. This enzyme works optimally at a pH of 4–5, remains stable at pH 2, and is completely resistant to digestion by pepsin ( 2 ). Furthermore, research has shown that Aspergillus niger prolyl endoprotease (AN-PEP) accelerates the breakdown of gluten in the stomach so much that very little gluten reaches the duodenum ( 3 ). This suggests that co-administration of AN-PEP with a meal containing gluten could potentially eliminate gluten toxicity, allowing people to (occasionally) abandon their strict gluten-free diet ( 3 ).
100% Tolerase® G Anti-Gluten Pills

Unique Enzyme Solution
Discover how our 100% Tolerase® G pills help digest gluten and support your lifestyle in a clear video.
Little Helpers & Tolerase® G: Stronger Together in the Fight against Gluten.
At Little Helpers we are proud of our unique partnership with the producer of Tolerase® G DSM . Together we have spent 2.5 years of research and worked tirelessly to develop and perfect our gluten digestion pill, which contains 100% Tolerase® G.
Our shared commitment to quality and effectiveness has resulted in a product that is unmatched in the market. We have chosen to manufacture locally in Europe to ensure the highest quality standards and control.
Our pill has proven to be many times more effective than our competitors, and we offer it at the best price. We are convinced that our pill can make a difference for people who struggle with gluten sensitivity. That is why we encourage everyone to try it and experience the benefits for themselves!
The Only Effective Anti-Gluten Pills
Little Helpers Anti-Gluten Pills contain 100% Tolerase® G. Tolerase® G is the only enzyme on the market that has been scientifically proven to be effective enough to break down gluten in the stomach to the point where the body no longer reacts to it.

Frequently Asked Questions
Our Expert
Drs. Ilse van Asperen
Drs. Ilse van Asperen is a medical doctor specialising in orthomolecular therapy and nutritional coaching. With a background in public and clinical health research and management, she focuses on addressing the root causes of health issues through diet, gut health, and stress reduction. As a professional editor for Little Helpers, she reviews all gluten-free content to ensure accuracy, safety, and scientific credibility. More about me
Our Sources
The information on this page is based on reputable health and nutrition organisations, ensuring accuracy and reliability for anyone following a gluten-free diet. For further details, see the sources below.
Sources
Experiences of our customers
Disclaimer: We do our best to provide accurate gluten information, but errors can happen. If you spot a mistake or have concerns, please contact us so we can make it right.