Is Inactive Yeast Gluten Free?
Inactive yeast itself is typically gluten-free, as it does not naturally contain gluten. However, it’s important to check the specific product label or with the manufacturer to ensure there hasn’t been any cross-contamination during processing or if any gluten-containing additives have been included.

Rich source of B-complex vitamins, especially B12 (in fortified varieties)
High in protein and essential amino acids
Contains minerals like zinc, selenium, and manganese
Good source of dietary fiber
Low in fat and calories
Provides antioxidants, particularly glutathione
May support immune system function
Can help maintain healthy skin, hair, and nails
Potentially beneficial for digestive health
May help lower cholesterol levels
Contains compounds that may have anti-inflammatory properties
Can be used as a flavor enhancer for those reducing salt intake
Suitable for vegetarians and vegans as a nutrient supplement
May help regulate blood sugar levels
Contains chromium, which may aid in glucose metabolismRemember that while inactive yeast offers these potential benefits, it’s always best to consult with a healthcare professional before making significant changes to your diet or supplement regimen.
People with yeast allergies: Those allergic to yeast should avoid all forms of yeast, including inactive yeast.
Individuals with inflammatory bowel disease (IBD): Some people with Crohn’s disease or ulcerative colitis may experience worsened symptoms when consuming yeast products.
Those with candida overgrowth: Although inactive yeast is not alive, some people with candida issues may prefer to avoid yeast-based products.
People on a low-FODMAP diet: Inactive yeast is high in mannitol, a type of FODMAP that can cause digestive issues in sensitive individuals.
Individuals taking certain medications: Inactive yeast may interact with some medications, such as MAO inhibitors used to treat depression.
People with gout: Yeast is high in purines, which can exacerbate gout symptoms in some individuals.
Those with autoimmune conditions: Some people with autoimmune disorders may be sensitive to yeast products.
Individuals on a low-sodium diet: Inactive yeast is often high in sodium and may not be suitable for those restricting salt intake.Always consult with a healthcare professional before making significant changes to your diet, especially if you have underlying health conditions or concerns.
Explore gluten management solutions like Tolerase® G.
What is Tolerase® G?
A new Approach for People with Gluten Sensitivity:
Explore the Science and Benefits of Tolerase® G.
Relief from Gluten Sensitivity
In the fight against gluten, there is a unique supplement that stands out: Little Helpers anti-gluten pills with 100% Tolerase® G.
This specific enzyme, Tolerase® G, a patented endopeptidase specific for proline, was developed by Royal DSM and has been scientifically proven to be effective in breaking down gluten residues ( 1) . Furthermore, research has shown that Aspergillus niger prolyl endoprotease (AN-PEP), an enzyme closely related to Tolerase® G, can break down gluten so effectively that it barely reaches the duodenum, potentially eliminating gluten toxicity ( 2) ( 3 ) .
On this page we explain why we are so enthusiastic about this supplement, supported by scientific research.

What is Gluten?
Gluten is a group of proteins found primarily in grains such as wheat, barley, and rye. These complex proteins give shape, strength, and texture to bread and other grain products. However, gluten is difficult to digest because it is rich in proline, a non-essential amino acid. This is why about 13% of the world’s population is sensitive to gluten in their diet (1).
How Tolerase® G Works
It is important to note that Tolerase G is designed to have optimal activity in the difficult conditions of the stomach. Its effectiveness on the digestion of gluten in the stomach and duodenum has been scientifically proven ( 1 ).
Studies have shown that a newly identified prolyl endoprotease derived from Aspergillus niger can effectively break down gluten proteins. This enzyme works optimally at a pH of 4–5, remains stable at pH 2, and is completely resistant to digestion by pepsin ( 2 ). Furthermore, research has shown that Aspergillus niger prolyl endoprotease (AN-PEP) accelerates the breakdown of gluten in the stomach so much that very little gluten reaches the duodenum ( 3 ). This suggests that co-administration of AN-PEP with a meal containing gluten could potentially eliminate gluten toxicity, allowing people to (occasionally) abandon their strict gluten-free diet ( 3 ).
100% Tolerase® G Anti-Gluten Pills

Unique Enzyme Solution
Discover how our 100% Tolerase® G pills help digest gluten and support your lifestyle in a clear video.
Little Helpers & Tolerase® G: Stronger Together in the Fight against Gluten.
At Little Helpers we are proud of our unique partnership with the producer of Tolerase® G DSM . Together we have spent 2.5 years of research and worked tirelessly to develop and perfect our gluten digestion pill, which contains 100% Tolerase® G.
Our shared commitment to quality and effectiveness has resulted in a product that is unmatched in the market. We have chosen to manufacture locally in Europe to ensure the highest quality standards and control.
Our pill has proven to be many times more effective than our competitors, and we offer it at the best price. We are convinced that our pill can make a difference for people who struggle with gluten sensitivity. That is why we encourage everyone to try it and experience the benefits for themselves!
The Only Effective Anti-Gluten Pills
Little Helpers Anti-Gluten Pills contain 100% Tolerase® G. Tolerase® G is the only enzyme on the market that has been scientifically proven to be effective enough to break down gluten in the stomach to the point where the body no longer reacts to it.

Frequently Asked Questions
Our Expert
Drs. Ilse van Asperen
Drs. Ilse van Asperen is a medical doctor specialising in orthomolecular therapy and nutritional coaching. With a background in public and clinical health research and management, she focuses on addressing the root causes of health issues through diet, gut health, and stress reduction. As a professional editor for Little Helpers, she reviews all gluten-free content to ensure accuracy, safety, and scientific credibility. More about me
Our Sources
The information on this page is based on reputable health and nutrition organisations, ensuring accuracy and reliability for anyone following a gluten-free diet. For further details, see the sources below.
Sources
Experiences of our customers
Disclaimer: We do our best to provide accurate gluten information, but errors can happen. If you spot a mistake or have concerns, please contact us so we can make it right.