Koji itself, which is a cultured mold (Aspergillus oryzae) grown on grains, is gluten-free. However, the substrate on which koji is grown can contain gluten if wheat or barley is used, so whether a koji product is gluten-free depends on the specific grain used in its production.

Rich in probiotics: Koji contains beneficial bacteria that can support gut health and improve digestion.
Enzyme production: It produces enzymes that can help break down proteins, carbohydrates, and fats, potentially aiding digestion.
Nutrient enhancement: Koji fermentation can increase the bioavailability of nutrients in foods, making them easier for the body to absorb.
Vitamin B production: It can produce various B vitamins, including B12, which is particularly beneficial for vegetarians and vegans.
Antioxidant properties: Koji contains compounds with antioxidant effects, which may help protect cells from damage caused by free radicals.
Potential immune system support: The probiotics in koji may help strengthen the immune system.
Blood sugar regulation: Some studies suggest that koji may help regulate blood sugar levels.
Potential anti-inflammatory effects: Certain compounds produced during koji fermentation may have anti-inflammatory properties.
Possible cholesterol-lowering effects: Some research indicates that koji may help reduce cholesterol levels.
Umami flavor enhancement: While not a direct health benefit, koji’s ability to enhance umami flavor can reduce the need for excessive salt in cooking.Remember that while these potential benefits are promising, more research is needed to fully understand and confirm many of koji’s health effects.
People with mold allergies: Koji is a type of mold, so those with mold allergies may experience adverse reactions.
Individuals with compromised immune systems: They may be more susceptible to infections from molds.
Those with severe yeast allergies: While koji is not a yeast, some people with severe yeast allergies may react to other fungi.
People taking certain medications: Koji can interact with some drugs, particularly those that affect blood clotting.
Pregnant women: As with many fermented foods, pregnant women should consult their doctor before consuming koji products.
Individuals with histamine intolerance: Fermented foods like koji can be high in histamines.
Those with specific gastrointestinal conditions: People with certain GI issues may need to avoid fermented foods.
Individuals on a low-salt diet: Some koji products, particularly miso, can be high in sodium.
People with specific dietary restrictions: Depending on the koji product, it may not be suitable for certain diets (e.g., gluten-free, low-FODMAP).Always consult with a healthcare professional if you have concerns about adding koji to your diet, especially if you have pre-existing health conditions or are taking medications.
Explore gluten management solutions like Tolerase® G.
What is Tolerase® G?
A new Approach for People with Gluten Sensitivity:
Explore the Science and Benefits of Tolerase® G.
Relief from Gluten Sensitivity
In the fight against gluten, there is a unique supplement that stands out: Little Helpers anti-gluten pills with 100% Tolerase® G.
This specific enzyme, Tolerase® G, a patented endopeptidase specific for proline, was developed by Royal DSM and has been scientifically proven to be effective in breaking down gluten residues ( 1) . Furthermore, research has shown that Aspergillus niger prolyl endoprotease (AN-PEP), an enzyme closely related to Tolerase® G, can break down gluten so effectively that it barely reaches the duodenum, potentially eliminating gluten toxicity ( 2) ( 3 ) .
On this page we explain why we are so enthusiastic about this supplement, supported by scientific research.

What is Gluten?
Gluten is a group of proteins found primarily in grains such as wheat, barley, and rye. These complex proteins give shape, strength, and texture to bread and other grain products. However, gluten is difficult to digest because it is rich in proline, a non-essential amino acid. This is why about 13% of the world’s population is sensitive to gluten in their diet (1).
How Tolerase® G Works
It is important to note that Tolerase G is designed to have optimal activity in the difficult conditions of the stomach. Its effectiveness on the digestion of gluten in the stomach and duodenum has been scientifically proven ( 1 ).
Studies have shown that a newly identified prolyl endoprotease derived from Aspergillus niger can effectively break down gluten proteins. This enzyme works optimally at a pH of 4–5, remains stable at pH 2, and is completely resistant to digestion by pepsin ( 2 ). Furthermore, research has shown that Aspergillus niger prolyl endoprotease (AN-PEP) accelerates the breakdown of gluten in the stomach so much that very little gluten reaches the duodenum ( 3 ). This suggests that co-administration of AN-PEP with a meal containing gluten could potentially eliminate gluten toxicity, allowing people to (occasionally) abandon their strict gluten-free diet ( 3 ).
100% Tolerase® G Anti-Gluten Pills

Unique Enzyme Solution
Discover how our 100% Tolerase® G pills help digest gluten and support your lifestyle in a clear video.
Little Helpers & Tolerase® G: Stronger Together in the Fight against Gluten.
At Little Helpers we are proud of our unique partnership with the producer of Tolerase® G DSM . Together we have spent 2.5 years of research and worked tirelessly to develop and perfect our gluten digestion pill, which contains 100% Tolerase® G.
Our shared commitment to quality and effectiveness has resulted in a product that is unmatched in the market. We have chosen to manufacture locally in Europe to ensure the highest quality standards and control.
Our pill has proven to be many times more effective than our competitors, and we offer it at the best price. We are convinced that our pill can make a difference for people who struggle with gluten sensitivity. That is why we encourage everyone to try it and experience the benefits for themselves!
The Only Effective Anti-Gluten Pills
Little Helpers Anti-Gluten Pills contain 100% Tolerase® G. Tolerase® G is the only enzyme on the market that has been scientifically proven to be effective enough to break down gluten in the stomach to the point where the body no longer reacts to it.

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Disclaimer: We do our best to provide accurate gluten information, but errors can happen. If you spot a mistake or have concerns, please contact us so we can make it right.