Is Lactose Monohydrate Gluten Free?
Yes, lactose monohydrate is gluten-free. Lactose monohydrate is a sugar derived from milk and does not contain any gluten proteins, which are found in wheat, barley, and rye.

Prebiotic function: Supports the growth of beneficial gut bacteria
Improved calcium absorption: May enhance calcium uptake in the intestines
Bone health support: Due to its role in calcium absorption, it may contribute to stronger bones
Digestive health: Can help maintain a healthy digestive system
Low glycemic index: Has a minimal impact on blood sugar levels
Carbohydrate source: Provides energy for the body
Potential anti-inflammatory effects: May help reduce inflammation in the gut
Improved mineral absorption: May enhance the absorption of other minerals besides calcium
Potential weight management aid: Low calorie content may support weight control efforts
Infant nutrition: Commonly used in infant formulas as a carbohydrate source
Potential immune system support: Through its effects on gut health and beneficial bacteriaIt’s important to note that while these benefits are associated with lactose monohydrate, individual responses may vary, and those with lactose intolerance should consult a healthcare professional before consuming products containing this ingredient.
People with lactose intolerance
Individuals with galactosemia (a rare genetic disorder affecting galactose metabolism)
Those with severe dairy allergies
Vegans who choose to avoid all animal-derived products
People following a strict dairy-free diet for personal or health reasons
Individuals with certain gastrointestinal conditions that may be aggravated by lactose consumption
Those who experience bloating, gas, or digestive discomfort after consuming lactose-containing products
Infants with congenital lactase deficiency
People adhering to certain religious or cultural dietary restrictions that prohibit dairy products
Those following a low FODMAP diet, as lactose is a FODMAP (fermentable oligosaccharides, disaccharides, monosaccharides, and polyols)It’s important to note that the degree of lactose sensitivity can vary among individuals, and some people may be able to tolerate small amounts of lactose. Always consult with a healthcare professional for personalized advice.
Explore gluten management solutions like Tolerase® G.
What is Tolerase® G?
A new Approach for People with Gluten Sensitivity:
Explore the Science and Benefits of Tolerase® G.
Relief from Gluten Sensitivity
In the fight against gluten, there is a unique supplement that stands out: Little Helpers anti-gluten pills with 100% Tolerase® G.
This specific enzyme, Tolerase® G, a patented endopeptidase specific for proline, was developed by Royal DSM and has been scientifically proven to be effective in breaking down gluten residues ( 1) . Furthermore, research has shown that Aspergillus niger prolyl endoprotease (AN-PEP), an enzyme closely related to Tolerase® G, can break down gluten so effectively that it barely reaches the duodenum, potentially eliminating gluten toxicity ( 2) ( 3 ) .
On this page we explain why we are so enthusiastic about this supplement, supported by scientific research.

What is Gluten?
Gluten is a group of proteins found primarily in grains such as wheat, barley, and rye. These complex proteins give shape, strength, and texture to bread and other grain products. However, gluten is difficult to digest because it is rich in proline, a non-essential amino acid. This is why about 13% of the world’s population is sensitive to gluten in their diet (1).
How Tolerase® G Works
It is important to note that Tolerase G is designed to have optimal activity in the difficult conditions of the stomach. Its effectiveness on the digestion of gluten in the stomach and duodenum has been scientifically proven ( 1 ).
Studies have shown that a newly identified prolyl endoprotease derived from Aspergillus niger can effectively break down gluten proteins. This enzyme works optimally at a pH of 4–5, remains stable at pH 2, and is completely resistant to digestion by pepsin ( 2 ). Furthermore, research has shown that Aspergillus niger prolyl endoprotease (AN-PEP) accelerates the breakdown of gluten in the stomach so much that very little gluten reaches the duodenum ( 3 ). This suggests that co-administration of AN-PEP with a meal containing gluten could potentially eliminate gluten toxicity, allowing people to (occasionally) abandon their strict gluten-free diet ( 3 ).
100% Tolerase® G Anti-Gluten Pills

Unique Enzyme Solution
Discover how our 100% Tolerase® G pills help digest gluten and support your lifestyle in a clear video.
Little Helpers & Tolerase® G: Stronger Together in the Fight against Gluten.
At Little Helpers we are proud of our unique partnership with the producer of Tolerase® G DSM . Together we have spent 2.5 years of research and worked tirelessly to develop and perfect our gluten digestion pill, which contains 100% Tolerase® G.
Our shared commitment to quality and effectiveness has resulted in a product that is unmatched in the market. We have chosen to manufacture locally in Europe to ensure the highest quality standards and control.
Our pill has proven to be many times more effective than our competitors, and we offer it at the best price. We are convinced that our pill can make a difference for people who struggle with gluten sensitivity. That is why we encourage everyone to try it and experience the benefits for themselves!
The Only Effective Anti-Gluten Pills
Little Helpers Anti-Gluten Pills contain 100% Tolerase® G. Tolerase® G is the only enzyme on the market that has been scientifically proven to be effective enough to break down gluten in the stomach to the point where the body no longer reacts to it.

Frequently Asked Questions
Our Expert
Drs. Ilse van Asperen
Drs. Ilse van Asperen is a medical doctor specialising in orthomolecular therapy and nutritional coaching. With a background in public and clinical health research and management, she focuses on addressing the root causes of health issues through diet, gut health, and stress reduction. As a professional editor for Little Helpers, she reviews all gluten-free content to ensure accuracy, safety, and scientific credibility. More about me
Our Sources
The information on this page is based on reputable health and nutrition organisations, ensuring accuracy and reliability for anyone following a gluten-free diet. For further details, see the sources below.
Sources
Experiences of our customers
Disclaimer: We do our best to provide accurate gluten information, but errors can happen. If you spot a mistake or have concerns, please contact us so we can make it right.