Generally speaking, pure mustard is gluten-free as it’s typically made from mustard seeds, vinegar, water, and spices, which do not contain gluten. However, some commercially prepared mustards may contain gluten-containing additives or be produced in facilities that also process gluten-containing products, so it’s always best to check the label or contact the manufacturer if you have celiac disease or gluten sensitivity.

Rich in antioxidants, particularly selenium and flavonoids
Contains anti-inflammatory compounds
May help reduce the risk of certain cancers, especially gastrointestinal cancers
Aids in digestion by stimulating the production of gastric juices
Provides a good source of omega-3 fatty acids
Contains glucosinolates, which may have anti-cancer properties
Helps boost metabolism and can aid in weight management
May help lower blood sugar levels
Good source of vitamins and minerals, including vitamin C, A, K, and B-complex vitamins
Contains calcium, which is beneficial for bone health
May help relieve congestion and respiratory issues
Has antibacterial properties that can help fight infections
Can act as a natural pain reliever for sore muscles and joints
May help improve cardiovascular health by reducing cholesterol levels
Contains fiber, which aids in digestive health and promotes feelings of fullnessRemember that these benefits can vary depending on the type of mustard and how it’s prepared. As with any food, it’s best to consume mustard as part of a balanced diet.
People with a mustard allergy: This is one of the most common food allergies and can cause severe reactions.
Those with sulfite sensitivity: Some mustards contain sulfites, which can trigger reactions in sensitive individuals.
Individuals with gastrointestinal issues: Mustard can irritate the digestive tract in some people with conditions like gastritis or acid reflux.
People on blood-thinning medications: Mustard seeds contain vitamin K, which can interfere with blood-thinning medications like warfarin.
Those with thyroid problems: Mustard contains goitrogens, which can interfere with thyroid function if consumed in large amounts.
Individuals with salicylate sensitivity: Mustard contains salicylates, which some people are sensitive to.
Young children: Due to its strong flavor and potential allergenicity, mustard is often introduced later in a child’s diet.
People on low-sodium diets: Some prepared mustards can be high in sodium.
Those with histamine intolerance: Mustard can be high in histamines, which some people have difficulty processing.Always consult with a healthcare professional if you have concerns about including mustard in your diet, especially if you have any health conditions or allergies.
Explore gluten management solutions like Tolerase® G.
What is Tolerase® G?
A new Approach for People with Gluten Sensitivity:
Explore the Science and Benefits of Tolerase® G.
Relief from Gluten Sensitivity
In the fight against gluten, there is a unique supplement that stands out: Little Helpers anti-gluten pills with 100% Tolerase® G.
This specific enzyme, Tolerase® G, a patented endopeptidase specific for proline, was developed by Royal DSM and has been scientifically proven to be effective in breaking down gluten residues ( 1) . Furthermore, research has shown that Aspergillus niger prolyl endoprotease (AN-PEP), an enzyme closely related to Tolerase® G, can break down gluten so effectively that it barely reaches the duodenum, potentially eliminating gluten toxicity ( 2) ( 3 ) .
On this page we explain why we are so enthusiastic about this supplement, supported by scientific research.

What is Gluten?
Gluten is a group of proteins found primarily in grains such as wheat, barley, and rye. These complex proteins give shape, strength, and texture to bread and other grain products. However, gluten is difficult to digest because it is rich in proline, a non-essential amino acid. This is why about 13% of the world’s population is sensitive to gluten in their diet (1).
How Tolerase® G Works
It is important to note that Tolerase G is designed to have optimal activity in the difficult conditions of the stomach. Its effectiveness on the digestion of gluten in the stomach and duodenum has been scientifically proven ( 1 ).
Studies have shown that a newly identified prolyl endoprotease derived from Aspergillus niger can effectively break down gluten proteins. This enzyme works optimally at a pH of 4–5, remains stable at pH 2, and is completely resistant to digestion by pepsin ( 2 ). Furthermore, research has shown that Aspergillus niger prolyl endoprotease (AN-PEP) accelerates the breakdown of gluten in the stomach so much that very little gluten reaches the duodenum ( 3 ). This suggests that co-administration of AN-PEP with a meal containing gluten could potentially eliminate gluten toxicity, allowing people to (occasionally) abandon their strict gluten-free diet ( 3 ).
100% Tolerase® G Anti-Gluten Pills

Unique Enzyme Solution
Discover how our 100% Tolerase® G pills help digest gluten and support your lifestyle in a clear video.
Little Helpers & Tolerase® G: Stronger Together in the Fight against Gluten.
At Little Helpers we are proud of our unique partnership with the producer of Tolerase® G DSM . Together we have spent 2.5 years of research and worked tirelessly to develop and perfect our gluten digestion pill, which contains 100% Tolerase® G.
Our shared commitment to quality and effectiveness has resulted in a product that is unmatched in the market. We have chosen to manufacture locally in Europe to ensure the highest quality standards and control.
Our pill has proven to be many times more effective than our competitors, and we offer it at the best price. We are convinced that our pill can make a difference for people who struggle with gluten sensitivity. That is why we encourage everyone to try it and experience the benefits for themselves!
The Only Effective Anti-Gluten Pills
Little Helpers Anti-Gluten Pills contain 100% Tolerase® G. Tolerase® G is the only enzyme on the market that has been scientifically proven to be effective enough to break down gluten in the stomach to the point where the body no longer reacts to it.

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