Nutritional yeast is typically gluten-free, as it’s made from a strain of yeast grown on molasses and does not contain wheat or other gluten-containing grains. However, it’s always best to check the specific product label or with the manufacturer to ensure the nutritional yeast is certified gluten-free, as cross-contamination can sometimes occur during processing.

Rich source of B vitamins, including B12 (in fortified varieties)
Complete protein containing all nine essential amino acids
Low in calories and fat
Good source of fiber
Contains minerals like zinc, selenium, and manganese
Naturally gluten-free
Provides a cheesy, nutty flavor for vegan and vegetarian dishes
May support immune function due to beta-glucans content
Potential to lower cholesterol levels
May help regulate blood sugar levels
Contains antioxidants like glutathione and selenomethionine
Supports healthy skin, hair, and nails
Can be used as a flavor enhancer in various recipes
Often fortified with additional nutrients
May have anti-inflammatory properties
Supports digestive health due to its fiber content
Can be a good nutritional supplement for vegetarians and vegans
May help in weight management due to its protein and fiber contentRemember that while nutritional yeast offers many benefits, it’s always best to consult with a healthcare professional before making significant changes to your diet or using it as a supplement.
People with inflammatory bowel diseases (IBD) like Crohn’s disease or ulcerative colitis, as it may exacerbate symptoms
Individuals with a yeast allergy or sensitivity
Those with a sensitivity to glutamic acid, which is naturally present in nutritional yeast
People prone to migraines, as some report nutritional yeast as a trigger
Individuals taking monoamine oxidase inhibitors (MAOIs), due to potential interactions
Those with gout or at high risk for gout, as nutritional yeast is high in purines
People on a low-tyramine diet, as nutritional yeast contains tyramine
Individuals with autoimmune conditions, who should consult their doctor before consuming it
Those with chronic candida infections, as there’s debate about whether it may exacerbate the condition
People with undiagnosed or uncontrolled high blood pressure, as nutritional yeast is often high in sodium
Individuals with phenylketonuria (PKU), due to the phenylalanine content in nutritional yeastAlways consult with a healthcare professional before making significant changes to your diet, especially if you have pre-existing health conditions.
Explore gluten management solutions like Tolerase® G.
What is Tolerase® G?
A new Approach for People with Gluten Sensitivity:
Explore the Science and Benefits of Tolerase® G.
Relief from Gluten Sensitivity
In the fight against gluten, there is a unique supplement that stands out: Little Helpers anti-gluten pills with 100% Tolerase® G.
This specific enzyme, Tolerase® G, a patented endopeptidase specific for proline, was developed by Royal DSM and has been scientifically proven to be effective in breaking down gluten residues ( 1) . Furthermore, research has shown that Aspergillus niger prolyl endoprotease (AN-PEP), an enzyme closely related to Tolerase® G, can break down gluten so effectively that it barely reaches the duodenum, potentially eliminating gluten toxicity ( 2) ( 3 ) .
On this page we explain why we are so enthusiastic about this supplement, supported by scientific research.

What is Gluten?
Gluten is a group of proteins found primarily in grains such as wheat, barley, and rye. These complex proteins give shape, strength, and texture to bread and other grain products. However, gluten is difficult to digest because it is rich in proline, a non-essential amino acid. This is why about 13% of the world’s population is sensitive to gluten in their diet (1).
How Tolerase® G Works
It is important to note that Tolerase G is designed to have optimal activity in the difficult conditions of the stomach. Its effectiveness on the digestion of gluten in the stomach and duodenum has been scientifically proven ( 1 ).
Studies have shown that a newly identified prolyl endoprotease derived from Aspergillus niger can effectively break down gluten proteins. This enzyme works optimally at a pH of 4–5, remains stable at pH 2, and is completely resistant to digestion by pepsin ( 2 ). Furthermore, research has shown that Aspergillus niger prolyl endoprotease (AN-PEP) accelerates the breakdown of gluten in the stomach so much that very little gluten reaches the duodenum ( 3 ). This suggests that co-administration of AN-PEP with a meal containing gluten could potentially eliminate gluten toxicity, allowing people to (occasionally) abandon their strict gluten-free diet ( 3 ).
100% Tolerase® G Anti-Gluten Pills

Unique Enzyme Solution
Discover how our 100% Tolerase® G pills help digest gluten and support your lifestyle in a clear video.
Little Helpers & Tolerase® G: Stronger Together in the Fight against Gluten.
At Little Helpers we are proud of our unique partnership with the producer of Tolerase® G DSM . Together we have spent 2.5 years of research and worked tirelessly to develop and perfect our gluten digestion pill, which contains 100% Tolerase® G.
Our shared commitment to quality and effectiveness has resulted in a product that is unmatched in the market. We have chosen to manufacture locally in Europe to ensure the highest quality standards and control.
Our pill has proven to be many times more effective than our competitors, and we offer it at the best price. We are convinced that our pill can make a difference for people who struggle with gluten sensitivity. That is why we encourage everyone to try it and experience the benefits for themselves!
The Only Effective Anti-Gluten Pills
Little Helpers Anti-Gluten Pills contain 100% Tolerase® G. Tolerase® G is the only enzyme on the market that has been scientifically proven to be effective enough to break down gluten in the stomach to the point where the body no longer reacts to it.

Frequently
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Disclaimer: We do our best to provide accurate gluten information, but errors can happen. If you spot a mistake or have concerns, please contact us so we can make it right.