Generally, plain pepperoni is gluten-free as it’s typically made from a combination of cured pork and beef mixed with spices, none of which contain gluten. However, it’s important to check the specific brand or manufacturer’s label, as some may add gluten-containing ingredients or process the pepperoni in facilities that handle gluten products, potentially leading to cross-contamination.

Protein source: Pepperoni contains protein, which is essential for building and repairing tissues in the body.
Vitamin B12: It provides vitamin B12, which is important for nerve function and the formation of red blood cells.
Zinc: Pepperoni contains zinc, which supports immune function and wound healing.
Iron: It provides iron, which is crucial for oxygen transport in the blood.
Selenium: Pepperoni contains selenium, an antioxidant that supports thyroid function and immune health.
Energy: As a high-fat food, it can provide energy for the body.
Flavor enhancer: Its strong flavor can make vegetables and other healthier foods more palatable for some people.It’s important to note that pepperoni is also high in saturated fat, sodium, and calories, so it should be consumed sparingly as part of a balanced diet. The potential negative health impacts of frequent consumption may outweigh these benefits for many people.
Those with pork allergies, as pepperoni is typically made from pork
Individuals following a halal or kosher diet, due to religious restrictions on pork consumption
Vegetarians and vegans
People with high cholesterol or heart disease, due to pepperoni’s high saturated fat content
Those on a low-sodium diet, as pepperoni is often high in salt
Individuals with nitrate sensitivity, as pepperoni often contains nitrates as preservatives
People with certain gastrointestinal conditions that are aggravated by spicy or fatty foods
Those trying to lose weight or maintain a low-calorie diet
Individuals with gout, as pepperoni is high in purines
People with migraines triggered by cured meats or nitrates
Those with histamine intolerance, as fermented foods like pepperoni can be high in histamines
Individuals with certain food additive sensitivities, as pepperoni may contain various preservatives and flavoringsRemember, if you have specific health concerns, it’s always best to consult with a healthcare professional or registered dietitian for personalized advice.
Explore gluten management solutions like Tolerase® G.
What is Tolerase® G?
A new Approach for People with Gluten Sensitivity:
Explore the Science and Benefits of Tolerase® G.
Relief from Gluten Sensitivity
In the fight against gluten, there is a unique supplement that stands out: Little Helpers anti-gluten pills with 100% Tolerase® G.
This specific enzyme, Tolerase® G, a patented endopeptidase specific for proline, was developed by Royal DSM and has been scientifically proven to be effective in breaking down gluten residues ( 1) . Furthermore, research has shown that Aspergillus niger prolyl endoprotease (AN-PEP), an enzyme closely related to Tolerase® G, can break down gluten so effectively that it barely reaches the duodenum, potentially eliminating gluten toxicity ( 2) ( 3 ) .
On this page we explain why we are so enthusiastic about this supplement, supported by scientific research.

What is Gluten?
Gluten is a group of proteins found primarily in grains such as wheat, barley, and rye. These complex proteins give shape, strength, and texture to bread and other grain products. However, gluten is difficult to digest because it is rich in proline, a non-essential amino acid. This is why about 13% of the world’s population is sensitive to gluten in their diet (1).
How Tolerase® G Works
It is important to note that Tolerase G is designed to have optimal activity in the difficult conditions of the stomach. Its effectiveness on the digestion of gluten in the stomach and duodenum has been scientifically proven ( 1 ).
Studies have shown that a newly identified prolyl endoprotease derived from Aspergillus niger can effectively break down gluten proteins. This enzyme works optimally at a pH of 4–5, remains stable at pH 2, and is completely resistant to digestion by pepsin ( 2 ). Furthermore, research has shown that Aspergillus niger prolyl endoprotease (AN-PEP) accelerates the breakdown of gluten in the stomach so much that very little gluten reaches the duodenum ( 3 ). This suggests that co-administration of AN-PEP with a meal containing gluten could potentially eliminate gluten toxicity, allowing people to (occasionally) abandon their strict gluten-free diet ( 3 ).
100% Tolerase® G Anti-Gluten Pills

Unique Enzyme Solution
Discover how our 100% Tolerase® G pills help digest gluten and support your lifestyle in a clear video.
Little Helpers & Tolerase® G: Stronger Together in the Fight against Gluten.
At Little Helpers we are proud of our unique partnership with the producer of Tolerase® G DSM . Together we have spent 2.5 years of research and worked tirelessly to develop and perfect our gluten digestion pill, which contains 100% Tolerase® G.
Our shared commitment to quality and effectiveness has resulted in a product that is unmatched in the market. We have chosen to manufacture locally in Europe to ensure the highest quality standards and control.
Our pill has proven to be many times more effective than our competitors, and we offer it at the best price. We are convinced that our pill can make a difference for people who struggle with gluten sensitivity. That is why we encourage everyone to try it and experience the benefits for themselves!
The Only Effective Anti-Gluten Pills
Little Helpers Anti-Gluten Pills contain 100% Tolerase® G. Tolerase® G is the only enzyme on the market that has been scientifically proven to be effective enough to break down gluten in the stomach to the point where the body no longer reacts to it.

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