Is Pesto Gluten Free?
Pesto itself is typically gluten-free, as its main ingredients (basil, olive oil, garlic, pine nuts, and Parmesan cheese) do not contain gluten. However, it’s important to check the specific brand or recipe, as some variations may include gluten-containing additives or be processed in facilities that handle gluten products.

Rich in antioxidants: Basil and olive oil contain antioxidants that help protect cells from damage.
Good source of healthy fats: Olive oil and pine nuts provide heart-healthy monounsaturated fats.
Anti-inflammatory properties: Basil has anti-inflammatory compounds that may help reduce inflammation in the body.
Vitamin K: Basil is high in vitamin K, which is important for blood clotting and bone health.
Immune system support: Garlic has immune-boosting properties and may help fight off infections.
Calcium and protein: Parmesan cheese provides calcium for bone health and protein for muscle maintenance.
May improve brain function: Some studies suggest that components in basil may enhance cognitive function.
Potential cancer-fighting properties: Certain compounds in basil and garlic have shown promise in cancer prevention research.
Heart health: The combination of olive oil and garlic may contribute to better cardiovascular health.
Digestive aid: Basil has traditionally been used to aid digestion and reduce bloating.Remember that these benefits are most pronounced when pesto is consumed in moderation as part of a balanced diet.
People with nut allergies (particularly pine nut allergies)
Those with severe dairy allergies (due to cheese content in traditional pesto)
Individuals on low-sodium diets (as pesto can be high in salt)
People with basil allergies
Those following a vegan diet (unless using a vegan pesto alternative)
Individuals on low-fat diets (as pesto is typically high in oils)
People with garlic allergies or intolerances
Those taking blood-thinning medications (due to high vitamin K content in basil)
Individuals with FODMAP sensitivities (due to garlic content)
People with severe gluten sensitivities (if the pesto contains breadcrumbs or was processed in a facility with wheat products)
Those following a strict keto diet (due to potential carb content from nuts and cheese)Remember, many of these concerns can be addressed with modified pesto recipes or by checking ingredients carefully. Always consult with a healthcare provider for personalized dietary advice.
Explore gluten management solutions like Tolerase® G.
What is Tolerase® G?
A new Approach for People with Gluten Sensitivity:
Explore the Science and Benefits of Tolerase® G.
Relief from Gluten Sensitivity
In the fight against gluten, there is a unique supplement that stands out: Little Helpers anti-gluten pills with 100% Tolerase® G.
This specific enzyme, Tolerase® G, a patented endopeptidase specific for proline, was developed by Royal DSM and has been scientifically proven to be effective in breaking down gluten residues ( 1) . Furthermore, research has shown that Aspergillus niger prolyl endoprotease (AN-PEP), an enzyme closely related to Tolerase® G, can break down gluten so effectively that it barely reaches the duodenum, potentially eliminating gluten toxicity ( 2) ( 3 ) .
On this page we explain why we are so enthusiastic about this supplement, supported by scientific research.

What is Gluten?
Gluten is a group of proteins found primarily in grains such as wheat, barley, and rye. These complex proteins give shape, strength, and texture to bread and other grain products. However, gluten is difficult to digest because it is rich in proline, a non-essential amino acid. This is why about 13% of the world’s population is sensitive to gluten in their diet (1).
How Tolerase® G Works
It is important to note that Tolerase G is designed to have optimal activity in the difficult conditions of the stomach. Its effectiveness on the digestion of gluten in the stomach and duodenum has been scientifically proven ( 1 ).
Studies have shown that a newly identified prolyl endoprotease derived from Aspergillus niger can effectively break down gluten proteins. This enzyme works optimally at a pH of 4–5, remains stable at pH 2, and is completely resistant to digestion by pepsin ( 2 ). Furthermore, research has shown that Aspergillus niger prolyl endoprotease (AN-PEP) accelerates the breakdown of gluten in the stomach so much that very little gluten reaches the duodenum ( 3 ). This suggests that co-administration of AN-PEP with a meal containing gluten could potentially eliminate gluten toxicity, allowing people to (occasionally) abandon their strict gluten-free diet ( 3 ).
100% Tolerase® G Anti-Gluten Pills

Unique Enzyme Solution
Discover how our 100% Tolerase® G pills help digest gluten and support your lifestyle in a clear video.
Little Helpers & Tolerase® G: Stronger Together in the Fight against Gluten.
At Little Helpers we are proud of our unique partnership with the producer of Tolerase® G DSM . Together we have spent 2.5 years of research and worked tirelessly to develop and perfect our gluten digestion pill, which contains 100% Tolerase® G.
Our shared commitment to quality and effectiveness has resulted in a product that is unmatched in the market. We have chosen to manufacture locally in Europe to ensure the highest quality standards and control.
Our pill has proven to be many times more effective than our competitors, and we offer it at the best price. We are convinced that our pill can make a difference for people who struggle with gluten sensitivity. That is why we encourage everyone to try it and experience the benefits for themselves!
The Only Effective Anti-Gluten Pills
Little Helpers Anti-Gluten Pills contain 100% Tolerase® G. Tolerase® G is the only enzyme on the market that has been scientifically proven to be effective enough to break down gluten in the stomach to the point where the body no longer reacts to it.

Frequently Asked Questions
Our Expert
Drs. Ilse van Asperen
Drs. Ilse van Asperen is a medical doctor specialising in orthomolecular therapy and nutritional coaching. With a background in public and clinical health research and management, she focuses on addressing the root causes of health issues through diet, gut health, and stress reduction. As a professional editor for Little Helpers, she reviews all gluten-free content to ensure accuracy, safety, and scientific credibility. More about me
Our Sources
The information on this page is based on reputable health and nutrition organisations, ensuring accuracy and reliability for anyone following a gluten-free diet. For further details, see the sources below.
Sources
Experiences of our customers
Disclaimer: We do our best to provide accurate gluten information, but errors can happen. If you spot a mistake or have concerns, please contact us so we can make it right.