Is Red 40 Gluten Free?
Yes, Red 40 is gluten-free. This artificial food coloring is not derived from any gluten-containing grains and is considered safe for people with celiac disease or gluten sensitivity.

Red 40 is not a nutritional supplement or ingredient with health benefits.
It is a synthetic dye used to add color to foods, beverages, and cosmetics.
The FDA has approved it for use in food products in the United States.
Some studies have suggested potential links between artificial food dyes, including Red 40, and: – Hyperactivity in children – Allergic reactions in some individuals – Potential carcinogenic effects (though evidence is limited and inconclusive)
Many health-conscious consumers and some health professionals recommend avoiding or limiting consumption of artificial food dyes, including Red 40.
Some countries have banned or restricted the use of Red 40 due to health concerns.
Natural alternatives like beetroot juice or other plant-based colorants are often considered healthier options for food coloring.In summary, Red 40 is not associated with health benefits, and its consumption is generally recommended to be limited or avoided by those concerned about potential health impacts of artificial food additives.
People with known allergies or sensitivities to Red 40
Individuals with asthma, as Red 40 may exacerbate symptoms in some cases
Those with attention deficit hyperactivity disorder (ADHD), as some studies suggest a possible link between artificial food dyes and increased hyperactivity
People with migraines or headaches that may be triggered by food additives
Individuals with autoimmune disorders, as some believe artificial food dyes may contribute to inflammation
Those following a natural or organic diet
People with a family history of adverse reactions to food dyes
Young children, as they may be more sensitive to the effects of artificial food dyes
Pregnant women, as a precautionary measure
Individuals with inflammatory bowel diseases, such as Crohn’s disease or ulcerative colitis
Those with a history of hives or urticaria that may be triggered by food additives
People trying to avoid artificial ingredients in their diet for general health reasonsIt’s important to note that while these groups may be advised to avoid Red 40, individual reactions can vary. Always consult with a healthcare professional for personalized advice.
Explore gluten management solutions like Tolerase® G.
What is Tolerase® G?
A new Approach for People with Gluten Sensitivity:
Explore the Science and Benefits of Tolerase® G.
Relief from Gluten Sensitivity
In the fight against gluten, there is a unique supplement that stands out: Little Helpers anti-gluten pills with 100% Tolerase® G.
This specific enzyme, Tolerase® G, a patented endopeptidase specific for proline, was developed by Royal DSM and has been scientifically proven to be effective in breaking down gluten residues ( 1) . Furthermore, research has shown that Aspergillus niger prolyl endoprotease (AN-PEP), an enzyme closely related to Tolerase® G, can break down gluten so effectively that it barely reaches the duodenum, potentially eliminating gluten toxicity ( 2) ( 3 ) .
On this page we explain why we are so enthusiastic about this supplement, supported by scientific research.

What is Gluten?
Gluten is a group of proteins found primarily in grains such as wheat, barley, and rye. These complex proteins give shape, strength, and texture to bread and other grain products. However, gluten is difficult to digest because it is rich in proline, a non-essential amino acid. This is why about 13% of the world’s population is sensitive to gluten in their diet (1).
How Tolerase® G Works
It is important to note that Tolerase G is designed to have optimal activity in the difficult conditions of the stomach. Its effectiveness on the digestion of gluten in the stomach and duodenum has been scientifically proven ( 1 ).
Studies have shown that a newly identified prolyl endoprotease derived from Aspergillus niger can effectively break down gluten proteins. This enzyme works optimally at a pH of 4–5, remains stable at pH 2, and is completely resistant to digestion by pepsin ( 2 ). Furthermore, research has shown that Aspergillus niger prolyl endoprotease (AN-PEP) accelerates the breakdown of gluten in the stomach so much that very little gluten reaches the duodenum ( 3 ). This suggests that co-administration of AN-PEP with a meal containing gluten could potentially eliminate gluten toxicity, allowing people to (occasionally) abandon their strict gluten-free diet ( 3 ).
100% Tolerase® G Anti-Gluten Pills

Unique Enzyme Solution
Discover how our 100% Tolerase® G pills help digest gluten and support your lifestyle in a clear video.
Little Helpers & Tolerase® G: Stronger Together in the Fight against Gluten.
At Little Helpers we are proud of our unique partnership with the producer of Tolerase® G DSM . Together we have spent 2.5 years of research and worked tirelessly to develop and perfect our gluten digestion pill, which contains 100% Tolerase® G.
Our shared commitment to quality and effectiveness has resulted in a product that is unmatched in the market. We have chosen to manufacture locally in Europe to ensure the highest quality standards and control.
Our pill has proven to be many times more effective than our competitors, and we offer it at the best price. We are convinced that our pill can make a difference for people who struggle with gluten sensitivity. That is why we encourage everyone to try it and experience the benefits for themselves!
The Only Effective Anti-Gluten Pills
Little Helpers Anti-Gluten Pills contain 100% Tolerase® G. Tolerase® G is the only enzyme on the market that has been scientifically proven to be effective enough to break down gluten in the stomach to the point where the body no longer reacts to it.

Frequently Asked Questions
Our Expert
Drs. Ilse van Asperen
Drs. Ilse van Asperen is a medical doctor specialising in orthomolecular therapy and nutritional coaching. With a background in public and clinical health research and management, she focuses on addressing the root causes of health issues through diet, gut health, and stress reduction. As a professional editor for Little Helpers, she reviews all gluten-free content to ensure accuracy, safety, and scientific credibility. More about me
Our Sources
The information on this page is based on reputable health and nutrition organisations, ensuring accuracy and reliability for anyone following a gluten-free diet. For further details, see the sources below.
Sources
Experiences of our customers
Disclaimer: We do our best to provide accurate gluten information, but errors can happen. If you spot a mistake or have concerns, please contact us so we can make it right.