Is Rennet Gluten Free?
Rennet itself is generally considered gluten-free, as it is an enzyme typically derived from animal stomachs or produced through microbial fermentation. However, it’s always important to check the specific product or brand of rennet being used, as some preparations or additives could potentially contain gluten-containing ingredients.

Aids digestion: Rennet contains enzymes that can help break down proteins, potentially aiding in digestion.
Calcium source: Cheeses made with rennet are often good sources of calcium, which is essential for bone health.
Protein content: Cheeses produced using rennet are typically high in protein, which is important for muscle growth and repair.
Vitamin B12: Some cheeses made with rennet contain vitamin B12, which is crucial for nerve function and the formation of red blood cells.
Probiotic potential: Some cheeses made with rennet may contain beneficial bacteria, which can support gut health.
Conjugated Linoleic Acid (CLA): Some dairy products, including certain cheeses, contain CLA, which may have anti-inflammatory properties.
Vitamin K2: Certain cheeses made with rennet can be sources of vitamin K2, which is important for bone and cardiovascular health.It’s important to note that these benefits are primarily associated with the cheese products made using rennet, rather than rennet itself. Additionally, the health impacts can vary depending on the type of cheese and individual dietary needs.
Strict vegetarians or vegans: Most traditional rennet is derived from the stomachs of young ruminants (usually calves), making it unsuitable for those who avoid animal products.
People with dairy allergies: While rennet itself is not a dairy product, it’s primarily used in cheese production, so those with dairy allergies should be cautious.
Individuals following kosher or halal diets: Unless specifically certified, rennet may not meet kosher or halal dietary requirements.
Those with specific enzyme sensitivities: Though rare, some individuals might have sensitivities to the enzymes in rennet.
People following a plant-based diet for health reasons: They may wish to avoid animal-derived rennet.
Individuals with ethical concerns about animal welfare: Some may object to the use of animal-derived rennet due to its source.
Those with lactose intolerance: While rennet itself doesn’t contain lactose, it’s used in cheese production, so lactose-intolerant individuals should be cautious with cheese containing rennet.Note that plant-based and microbial alternatives to traditional rennet are available, which may be suitable for some of these groups.
Explore gluten management solutions like Tolerase® G.
What is Tolerase® G?
A new Approach for People with Gluten Sensitivity:
Explore the Science and Benefits of Tolerase® G.
Relief from Gluten Sensitivity
In the fight against gluten, there is a unique supplement that stands out: Little Helpers anti-gluten pills with 100% Tolerase® G.
This specific enzyme, Tolerase® G, a patented endopeptidase specific for proline, was developed by Royal DSM and has been scientifically proven to be effective in breaking down gluten residues ( 1) . Furthermore, research has shown that Aspergillus niger prolyl endoprotease (AN-PEP), an enzyme closely related to Tolerase® G, can break down gluten so effectively that it barely reaches the duodenum, potentially eliminating gluten toxicity ( 2) ( 3 ) .
On this page we explain why we are so enthusiastic about this supplement, supported by scientific research.

What is Gluten?
Gluten is a group of proteins found primarily in grains such as wheat, barley, and rye. These complex proteins give shape, strength, and texture to bread and other grain products. However, gluten is difficult to digest because it is rich in proline, a non-essential amino acid. This is why about 13% of the world’s population is sensitive to gluten in their diet (1).
How Tolerase® G Works
It is important to note that Tolerase G is designed to have optimal activity in the difficult conditions of the stomach. Its effectiveness on the digestion of gluten in the stomach and duodenum has been scientifically proven ( 1 ).
Studies have shown that a newly identified prolyl endoprotease derived from Aspergillus niger can effectively break down gluten proteins. This enzyme works optimally at a pH of 4–5, remains stable at pH 2, and is completely resistant to digestion by pepsin ( 2 ). Furthermore, research has shown that Aspergillus niger prolyl endoprotease (AN-PEP) accelerates the breakdown of gluten in the stomach so much that very little gluten reaches the duodenum ( 3 ). This suggests that co-administration of AN-PEP with a meal containing gluten could potentially eliminate gluten toxicity, allowing people to (occasionally) abandon their strict gluten-free diet ( 3 ).
100% Tolerase® G Anti-Gluten Pills

Unique Enzyme Solution
Discover how our 100% Tolerase® G pills help digest gluten and support your lifestyle in a clear video.
Little Helpers & Tolerase® G: Stronger Together in the Fight against Gluten.
At Little Helpers we are proud of our unique partnership with the producer of Tolerase® G DSM . Together we have spent 2.5 years of research and worked tirelessly to develop and perfect our gluten digestion pill, which contains 100% Tolerase® G.
Our shared commitment to quality and effectiveness has resulted in a product that is unmatched in the market. We have chosen to manufacture locally in Europe to ensure the highest quality standards and control.
Our pill has proven to be many times more effective than our competitors, and we offer it at the best price. We are convinced that our pill can make a difference for people who struggle with gluten sensitivity. That is why we encourage everyone to try it and experience the benefits for themselves!
The Only Effective Anti-Gluten Pills
Little Helpers Anti-Gluten Pills contain 100% Tolerase® G. Tolerase® G is the only enzyme on the market that has been scientifically proven to be effective enough to break down gluten in the stomach to the point where the body no longer reacts to it.

Frequently
Asked Questions 1
Disclaimer: We do our best to provide accurate gluten information, but errors can happen. If you spot a mistake or have concerns, please contact us so we can make it right.