Is Seitan Gluten Free?
No, seitan is not gluten-free. In fact, seitan is made primarily from wheat gluten, which is the main protein found in wheat.

High in protein: Seitan is an excellent source of plant-based protein, containing about 25 grams per 100 grams.
Low in fat: It is naturally low in fat, making it a good option for those watching their fat intake.
Low in calories: Seitan is relatively low in calories compared to many animal-based protein sources.
Contains essential amino acids: While not a complete protein, seitan contains most of the essential amino acids our bodies need.
Good source of iron: Seitan provides a significant amount of iron, which is important for oxygen transport in the body.
Cholesterol-free: As a plant-based food, seitan contains no cholesterol.
May aid in weight management: Its high protein content can help promote feelings of fullness and satiety.
Versatile in cooking: Seitan can be prepared in many ways, making it easier to maintain a varied, healthy diet.
Contains selenium: Seitan provides selenium, an important antioxidant that supports immune function.
May help reduce risk of heart disease: As a plant-based protein, it can be part of a heart-healthy diet.
Good for muscle building: Its high protein content makes it beneficial for those looking to build or maintain muscle mass.Remember that seitan is not suitable for people with celiac disease or gluten sensitivity, as it is made primarily from wheat gluten.
People with celiac disease
Individuals with non-celiac gluten sensitivity
Those with wheat allergies
People following a gluten-free diet for medical reasons
Individuals with inflammatory bowel diseases (IBD) who are sensitive to gluten
Those following specific diets that exclude wheat or gluten (e.g., paleo, keto)
People with certain autoimmune conditions who may be advised to avoid gluten
Individuals trying to reduce their intake of processed foods (as some commercial seitan products can be highly processed)
Those with digestive issues that are exacerbated by consuming gluten or wheat products
People following certain religious dietary restrictions that prohibit wheat consumptionIt’s important to note that if you’re unsure about whether you should consume seitan, it’s best to consult with a healthcare professional or registered dietitian for personalized advice.
Explore gluten management solutions like Tolerase® G.
What is Tolerase® G?
A new Approach for People with Gluten Sensitivity:
Explore the Science and Benefits of Tolerase® G.
Relief from Gluten Sensitivity
In the fight against gluten, there is a unique supplement that stands out: Little Helpers anti-gluten pills with 100% Tolerase® G.
This specific enzyme, Tolerase® G, a patented endopeptidase specific for proline, was developed by Royal DSM and has been scientifically proven to be effective in breaking down gluten residues ( 1) . Furthermore, research has shown that Aspergillus niger prolyl endoprotease (AN-PEP), an enzyme closely related to Tolerase® G, can break down gluten so effectively that it barely reaches the duodenum, potentially eliminating gluten toxicity ( 2) ( 3 ) .
On this page we explain why we are so enthusiastic about this supplement, supported by scientific research.

What is Gluten?
Gluten is a group of proteins found primarily in grains such as wheat, barley, and rye. These complex proteins give shape, strength, and texture to bread and other grain products. However, gluten is difficult to digest because it is rich in proline, a non-essential amino acid. This is why about 13% of the world’s population is sensitive to gluten in their diet (1).
How Tolerase® G Works
It is important to note that Tolerase G is designed to have optimal activity in the difficult conditions of the stomach. Its effectiveness on the digestion of gluten in the stomach and duodenum has been scientifically proven ( 1 ).
Studies have shown that a newly identified prolyl endoprotease derived from Aspergillus niger can effectively break down gluten proteins. This enzyme works optimally at a pH of 4–5, remains stable at pH 2, and is completely resistant to digestion by pepsin ( 2 ). Furthermore, research has shown that Aspergillus niger prolyl endoprotease (AN-PEP) accelerates the breakdown of gluten in the stomach so much that very little gluten reaches the duodenum ( 3 ). This suggests that co-administration of AN-PEP with a meal containing gluten could potentially eliminate gluten toxicity, allowing people to (occasionally) abandon their strict gluten-free diet ( 3 ).
100% Tolerase® G Anti-Gluten Pills

Unique Enzyme Solution
Discover how our 100% Tolerase® G pills help digest gluten and support your lifestyle in a clear video.
Little Helpers & Tolerase® G: Stronger Together in the Fight against Gluten.
At Little Helpers we are proud of our unique partnership with the producer of Tolerase® G DSM . Together we have spent 2.5 years of research and worked tirelessly to develop and perfect our gluten digestion pill, which contains 100% Tolerase® G.
Our shared commitment to quality and effectiveness has resulted in a product that is unmatched in the market. We have chosen to manufacture locally in Europe to ensure the highest quality standards and control.
Our pill has proven to be many times more effective than our competitors, and we offer it at the best price. We are convinced that our pill can make a difference for people who struggle with gluten sensitivity. That is why we encourage everyone to try it and experience the benefits for themselves!
The Only Effective Anti-Gluten Pills
Little Helpers Anti-Gluten Pills contain 100% Tolerase® G. Tolerase® G is the only enzyme on the market that has been scientifically proven to be effective enough to break down gluten in the stomach to the point where the body no longer reacts to it.

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Disclaimer: We do our best to provide accurate gluten information, but errors can happen. If you spot a mistake or have concerns, please contact us so we can make it right.